Composite

Part:BBa_K190036

Designed by: Michael Verhoeven   Group: iGEM09_Groningen   (2009-09-07)


GVP with LacI regulated promoter

The Gas Vesicle gene cluster (BBa_I750016) submitted by Melbourne 2007 team and the LacI and CAP regulated promoter BBa_R0010 were combined, and the product ligated into vector pSB1AC3. The construct was used to test and caracterize the gas vesicle formation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 542
    Illegal BamHI site found at 4347
    Illegal XhoI site found at 4035
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 993
    Illegal NgoMIV site found at 1395
    Illegal AgeI site found at 4314
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2805
    Illegal SapI.rc site found at 3961


[edit]
Categories
Parameters
biology
controlIPTG, lacI
directionForward
negative_regulators1
o_h
o_l
positive_regulators